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Test Code LAB260 Chlamydia trachomatis, PCR

Performing Lab

Arnot Ogden Medical Center

Ordering Recommendations

Detection of Chlamydia trachomatis.

Collect

Urine (First Catch or Dirty) (Male and Female patients)

Xpert CT/NG Swab

  • self collect vaginal
  • endocervical Swab
  • rectal
  • pharyngeal
     

Collection Device

Urine: Sterile Container

Swab: Xpert CT/NG Swab Collection Kit (MUST use swab provided in kit)

Specimen Volume

Urine: first catch: 20-50 mL

Stability (from collection to initiation)

  Room Temperature Refrigerated
First Catch Urine (femle) in sterile cup 24 hours 8 days
First Catch Urine (male) in a sterile cup 3 days 8 days
Xpert Swab Kit 60 days 60 days

 

Storage/Transport Temperature

Room Temperature or Refrigerated

Minimum Requirements

Urine: 7mL

Methodology

Real-Time PCR

Start Date

9/13/2017

Last Review Date

6/19/2025

Specimen Handling

Room Temperature or Refrigerated

Reported

Same shift

Specimen Preparation

Urine

  • Patient should not have urinated for at least 1 hour prior to collection.
  • Patients should not cleanse the labia or penis prior to collection.
  • Collect the first 20-50 mL of the initial urine stream into a sterile urine cup.
  • Collecting a larger volume may reduce test sensitivity.

 

Xpert CT/NG Vaginal/Endocervical Swabs

  • Remove excess mucus from cervix and surrounding area using the large individually wrapped cleaning swab.
  • Open the package that contains the pink capped Xpert swab reagent transport tube and individually wrapped collection swab.
  • Open the collection swab wrapper and hold swab in the middle of the shaft between your thumb and forefinger.
  • Insert the collection swab into the endocervical canal and rotate swab for 10-30 seconds.
  • Withdraw the collection swab from endocervical canal.
  • Unscrew cap from the transport tube and immediately insert the collection swab into the tube.
  • Break the swab shaft against the side of the tube at the score mark.
  • Replace the pink cap back on the reagent tube and secure tightly.

XpertCT/NG Pharyngeal Swab

  • Open the collection kit package and discard the larger swab.
  • Open the collection swab by peeling down from the top.
  • Remove the swab, taking care not to touch the top or lay it down.
  • Hold the swab in your hand, placing the thumb and forefinger in the middle of the shaft.
  • Instruct the patient to open the mouth widely. Position the tongue toward the bottom of the mouth. Swab areas of the pharynx (tonsil, posterior wall, uvula, posterior wall).
  • While holding the swab, unscrew the top from the transport tube.
  • Break the shaft of the swab on the scoreline. Avoid splashing tube contents on your skin.
  • Recap the tube.


XpertCT/NG Rectal Swab

  • Open the collection kit package and discard the larger swab.
  • Open the collection swab by peeling down from the top.
  • Remove the swab, taking care not to touch the top or lay it down.
  • Hold the swab in your hand, placing the thumb and forefinger in the middle of the shaft.
  • Carefully insert the swab approximately 1 cm beyond the anal sphincter and rotate gently.
  • While holding the swab, unscrew the top from the transport tube.
  • Break the shaft of the swab on the scoreline. Avoid splashing tube contents on your skin.
  • Recap the tube.

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