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Test Code LAB913 Cytomegalovirus (CMV) DNA Detection and Quantification by Real-Time PCR, Plasma


Shipping Instructions


1. Ship specimen frozen on dry ice only.

2. If shipment will be delayed for more than 24 hours, freeze plasma at -20 to -80° C (up to 84 days) until shipment on dry ice.



Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Lavender top (EDTA)

Submission Container/Tube: Plastic vial

Specimen Volume: 1.5 mL

Collection Instructions:

1. Centrifuge blood collection tube and aliquot plasma into a plastic vial per manufacturer's instructions (eg, centrifuge and aliquot within 2 hours of collection for BD Vacutainer tubes).

2. Freeze aliquoted plasma for shipment.


Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-Microbiology Test Request (T244)

-Renal Diagnostics Test Request (T830)

-General Test Request (T239)

Secondary ID

601954

Useful For

Detection and quantification of cytomegalovirus (CMV) viremia

 

Monitoring CMV disease progression and response to antiviral therapy

Method Name

Real-Time Polymerase Chain Reaction (RT-PCR)

Reporting Name

CMV DNA Detect/Quant, P

Specimen Type

Plasma EDTA

Specimen Minimum Volume

0.6 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma EDTA Frozen (preferred) 84 days
  Refrigerated  6 days

Reject Due To

Gross hemolysis Reject
Gross lipemia OK
Gross icterus OK

Reference Values

Undetected

Cautions

Cytomegalovirus (CMV) viral load results generated with this assay may be higher (up to 1.00 log IU/mL) than those from the previous cobas AmpliPrep/cobas TaqMan CMV test (Roche Molecular Systems Inc), due to differences in the sensitivity of both assays.

 

Variants within the highly conserved regions of the CMV DNA polymerase (UL54) gene covered by cobas CMV may affect primers or probe binding resulting in the under quantitation of virus or failure to detect the presence of virus. The cobas CMV assay mitigates this risk by use of redundant CMV target sequence amplification primers.

Day(s) Performed

Monday through Saturday

Report Available

1 to 5 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

87497

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CMVQN CMV DNA Detect/Quant, P 72493-0

 

Result ID Test Result Name Result LOINC Value
601954 CMV DNA Detect/Quant, P 72493-0

NY State Approved

Yes