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HelpTest Code LAB860 Lyme Disease Antibody, Immunoblot, Serum
Reporting Name
Lyme Disease Ab, Immunoblot, SUseful For
Aiding in the diagnosis of systemic Lyme disease
This test should not be used as a screening assay.
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
SerumSpecimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume:0.75 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 14 days |
| Frozen | 30 days |
Reference Values
IgG: Negative
IgM: Negative
Reference values apply to all ages
Day(s) Performed
Monday, Wednesday, Friday
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86617 x 2
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| LYWB | Lyme Disease Ab, Immunoblot, S | 18203-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 5744 | IgG Immunoblot | 6320-6 |
| 2992 | IgG detected against: | 13502-0 |
| 23931 | IgM Immunoblot | 6321-4 |
| 23932 | IgM detected against: | 13503-8 |
| 6241 | Interpretation | 12781-1 |
Testing Algorithm
For information see Acute Tick-Borne Disease Testing Algorithm.
Cautions
The immunoblot result may be negative in specimens that are weakly positive by enzyme immunoassay or in patients with early Lyme disease.
Test results should be used in conjunction with clinical evaluation and information related to tick exposure.
A negative test result does not necessarily rule out current or recent infection. The specimen may have been collected before demonstrable antibody developed. Patients with early disease often have serum antibody titers below the diagnostic threshold for several weeks following disease onset.
Test results from pregnant women or patients who are immunosuppressed may be difficult to interpret.
Positive test results may not be valid in persons who have received blood or blood product transfusions within the past several months.
Antibiotic therapy administered early following exposure or disease onset may suppress the antibody response to the point that diagnostic threshold levels are never attained.
Lyme disease serology should not be used for monitoring treatment response, as IgG can remain detectable for years post-resolution of infection.
False-positive reactions may occur with patients with other spirochetal diseases (syphilis, yaws, pinta, relapsing fever, or leptospirosis), recent Epstein-Barr virus infection (ie, infectious mononucleosis), influenza, autoimmune disorders (eg, present of extractable nuclear antigens), multiple sclerosis, or amyotrophic lateral sclerosis.
Report Available
Same day/1 to 4 daysReject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
| Heat-inactivated specimen | Reject |
NY State Approved
YesMethod Name
Immunoblot Microarray
Forms
If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request with the specimen.