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Test Code LAB458 Cytoplasmic Neutrophil Antibodies, Serum

Reporting Name

Cytoplasmic Neutrophilic Ab, S

Useful For

Evaluating patients with clinical features of ANCA-associated vasculitis, specifically granulomatosus with polyangiitis, microscopic polyangiitis, and eosinophilic granulomatosus with polyangiitis

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum


Additional Testing Requirements


When used for diagnosis, it is recommended that specific tests for proteinase 3 antibodies and myeloperoxidase antibodies be performed first, with additional testing for anti-neutrophil cytoplasmic antibodies only needed in certain circumstances.(3) A testing algorithm based on these recommendations is available. For more information see VASC / Antineutrophil Cytoplasmic Antibodies Vasculitis Panel, Serum.



Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.8 mL

Collection Instructions: Centrifuge and aliquot serum into plastic vial.


Specimen Minimum Volume

0.4 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 21 days
  Frozen  21 days

Reference Values

<1:4 (Negative)

Day(s) Performed

Monday through Saturday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

86036 x2

86037-Titer (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ANCA Cytoplasmic Neutrophilic Ab, S 87427-1

 

Result ID Test Result Name Result LOINC Value
3114 c-ANCA In Process
3119 p-ANCA 17357-5

Cautions

A positive result for proteinase 3 (PR3)- anti-neutrophil cytoplasmic antibody (ANCA), myeloperoxidase (MPO)-ANCA, or ANCA by IIF is not diagnostic for any ANCA-associated vasculitis and must be interpreted in the clinical context of the patient.

 

Negative results for PR3-ANCA, MPO-ANCA, and ANCA by indirect immunofluorescence (IIF) do not exclude the possibility of ANCA-associated vasculitis.

 

For monitoring disease activity, suggest ordering PR3 / Proteinase 3 Antibodies, Serum or MPO / Myeloperoxidase Antibodies, IgG, Serum

 

Antibodies specific for antigens other than PR3 and MPO may lead to nuclear, perinuclear or cytoplasmic staining on ethanol-fixed neutrophils. A positive or indeterminate pANCA or cANCA by IIF in the absence of a detectable PR3-ANCA or MPO-ANCA by antigen-specific immunoassay may indicate the presence of an antibody of unidentified specificity.

 

If ordered as a stand-alone test, all positive ANCA by IIF results should be confirmed for PR3-ANCA MPO-ANCA by solid-phase immunoassays.

Report Available

3 to 4 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus OK
Heat-treated specimen Reject

NY State Approved

Yes

Method Name

Indirect Immunofluorescence (IIF)

Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-General Request (T239)

-Renal Diagnostics Test Request (T830)

Secondary ID

9441