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Test Code LAB3244 Sickle Solubility, Blood

Reporting Name

Sickle Solubility, B

Useful For

Screening for presence or absence of hemoglobin (Hb) S (sickle cell disease)

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Whole Blood EDTA


Ordering Guidance


This is a screening test only. For quantification of hemoglobin S, order HBEL1 / Hemoglobin Electrophoresis Evaluation, Blood.



Necessary Information


1. Patient's age is required.

2. Include recent transfusion information.



Specimen Required


Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD solution B), green top (heparin)

Specimen Volume: 1 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send whole blood specimen in the original tube. Do not aliquot.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Whole Blood EDTA Refrigerated 14 days

Reference Values

Negative

Day(s) Performed

Monday through Friday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

85660

LOINC Code Information

Test ID Test Order Name Order LOINC Value
SDEX Sickle Solubility, B 6864-3

 

Result ID Test Result Name Result LOINC Value
9180 Sickle Solubility, B 6864-3

Cautions

A positive test is presumptive evidence for hemoglobin (Hb) S (sickle cell disease). However, rare sickling Hbs such as Hb C-Harlem (C-Georgetown) and Hb I will also produce a positive result.

 

This test only detects the presence of Hb S. It cannot differentiate sickle cell trait (heterozygous Hb S) from sickle cell disease (homozygous Hb S), or Hb S in combination with other abnormalities (eg, S/C, S/D, S/G, S/E, S/beta-thalassemia, S/O-Arab, S/New York, and C-Georgetown trait).

 

The use of packed red blood cells (RBC) instead of whole blood significantly reduces false negatives due to anemia and false positives due to hypergammaglobulinemia (eg, multiple myeloma).

 

False positives can occur due to large numbers of nucleated RBC.

 

False negatives can occur due to an insufficient quantity of Hb S due to age (neonates) or transfusion. Hb S concentrations 15% to 20% or less may give a negative result.

Report Available

1 to 4 days

Reject Due To

Gross hemolysis Reject

NY State Approved

Yes

Method Name

Hemoglobin S Solubility

Forms

If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen.

Secondary ID

9180