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Test Code LAB3210 Antineutrophil Cytoplasmic Antibodies Vasculitis Panel, Serum

Reporting Name

ANCA Panel for Vasculitis, S

Useful For

Evaluating patients with clinical features of anti-neutrophil cytoplasmic antibody-associated vasculitis, specifically granulomatosis with polyangiitis, microscopic polyangiitis, and eosinophilic granulomatosis with polyangiitis

Profile Information

Test ID Reporting Name Available Separately Always Performed
MPO Myeloperoxidase Ab, S Yes Yes
PR3 Proteinase 3 Ab (PR3), S Yes Yes

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
ANCA Cytoplasmic Neutrophilic Ab, S Yes No

Testing Algorithm

If either the myeloperoxidase antibody or proteinase 3 antibody result is greater than or equal to 0.4 U, then cytoplasmic neutrophilic antibody testing will be performed at an additional charge.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum


Ordering Guidance


For monitoring disease activity, order either PR3 / Proteinase 3 Antibodies, IgG, Serum or MPO / Myeloperoxidase Antibodies, IgG, Serum.



Specimen Required


Supplies: Sarstedt Aliquot Tube 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 21 days
  Frozen  21 days

Reference Values

MYELOPEROXIDASE ANTIBODIES, IgG

<0.4 U (negative)

0.4-0.9 U (equivocal)

≥1.0 U (positive) 

Reference values apply to all ages.

 

PROTEINASE 3 ANTIBODIES, IgG

<0.4 U (negative)

0.4-0.9 U (equivocal)

≥1.0 U (positive) 

Reference values apply to all ages.

Day(s) Performed

Monday through Saturday

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

83516 x 2

86036 x 2 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
VASC ANCA Panel for Vasculitis, S 90230-4

 

Result ID Test Result Name Result LOINC Value
MPO Myeloperoxidase Ab, S 48404-8
PR3 Proteinase 3 Ab (PR3), S 74106-6

Cautions

A positive result for proteinase 3 (PR3)-anti-neutrophil cytoplasmic antibodies (ANCA), myeloperoxidase (MPO)-ANCA, or ANCA by indirect immunofluorescence (IIF) is not diagnostic for any ANCA-associated vasculitis and must be interpreted in the clinical context of the patient.

 

Negative results for PR3-ANCA, MPO ANCA, and ANCA by IIF do not exclude the possibility of ANCA-associated vasculitis.

 

The recommended testing to monitor disease activity is either PR3 / Proteinase 3 Antibodies, IgG, Serum or MPO / Myeloperoxidase Antibodies, IgG, Serum

 

Antibodies specific for antigens other than PR3 and MPO may lead to nuclear, perinuclear, or cytoplasmic staining on ethanol-fixed neutrophils. A positive or indeterminate pANCA or cANCA by IIF in the absence of a detectable PR3-ANCA or MPO-ANCA by antigen-specific immunoassay may indicate the presence of an antibody of unidentified specificity.

Report Available

3 to 4 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus OK

NY State Approved

Yes

Method Name

Multiplex Flow Immunoassay

Secondary ID

83012

Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-Renal Diagnostics Test Request (T830)

-General Test Request (T239)