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Test Code LAB274 Direct Coombs

Performing Lab

Arnot Ogden Medical Center/ Ira Davenport Memorial Hospital

Ordering Recommendations

Demonstrating in vivo coating of RBCs with IgG or the complement component C3d in the following settings:
-Autoimmune hemolytic anemia
-Hemolytic transfusion reactions
-Drug-induced hemolytic anemia

Collect

Whole Blood

Collection Device

EDTA Pink Tube or lavender microtainer for pediatric draws

Stability (from collection to initiation)

Room Temp 24 hours
Refrigerated 24 hours

Storage/Transport Temperature

Room Temp or Refrigerated

Specimen Volume

5 ml

1 ml for pediatric patients

Minimum Requirements

3 mL

Remarks

TURN AROUND TIME

STAT 1 hour
Routine 8 hours

Synonyms

Direct Antiglobulin Test

Methodology

Gel Column

Start Date

9/13/2017

Last Review Date

06/20/2025

7/11/25 AP