Sign in →

Test Code LAB1374 BK Virus DNA Detection and Quantification, Plasma


Shipping Instructions


1. Ship specimen frozen on dry ice only.

2. If shipment will be delayed for more than 24 hours, freeze plasma at -20 to -80° C (up to 84 days) until shipment on dry ice.



Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Lavender top (EDTA)

Submission Container/Tube: Plastic vial

Specimen Volume: 1.5 mL

Collection Instructions:

1. Centrifuge blood collection tube and aliquot into a plastic vial per manufacturer's instructions (eg, centrifuge within 2 hours of collection for BD Vacutainer tubes).

2. Freeze aliquoted plasma for shipment.


Secondary ID

614567

Useful For

Detection and serial monitoring of BK virus-associated nephropathy in kidney transplant recipients using plasma specimens

 

Detection and serial monitoring of BK virus-associated hemorrhagic cystitis in organ transplant recipients

Method Name

Real-Time Polymerase Chain Reaction (PCR)

Reporting Name

BKV DNA Detect/Quant, P

Specimen Type

Plasma EDTA

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma EDTA Frozen (preferred) 84 days
  Refrigerated  6 days

Reject Due To

Gross hemolysis Reject
Gross lipemia OK
Gross icterus OK

Reference Values

Undetected

Cautions

On average, quantitative BK virus (BKV) DNA results in plasma tested with this assay can be up to 1.4-fold (about 0.15 log IU/mL) higher than those generated from the previous laboratory-developed BKV DNA quantification assay performed at Mayo Clinic Laboratories, due to differences in the specimen extraction method and design in the amplification primers and probes for the viral target sequences.

 

A single "Undetected" test result does not necessarily rule out the presence BKV infection or reactivation. Serial measurement (eg, once weekly) of BKV DNA in plasma is recommended to determine the BKV replication status in a given transplant recipient.

Day(s) Performed

Monday through Saturday

Report Available

1 to 3 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

87799

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PBKQN BKV DNA Detect/Quant, P 32284-2

 

Result ID Test Result Name Result LOINC Value
614567 BKV DNA Detect/Quant, P 32284-2

NY State Approved

Yes

Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-General Request (T239)

-Microbiology Test Request (T244)

-Renal Diagnostics Test Request (T830)