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Test Code LAB1054 Methadone and Metabolites, Serum

Useful For

Compliance monitoring of methadone

 

Assessment of methadone toxicity

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Methadone and Metabolite, S

Specimen Type

Serum Red


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Red top (Serum gel/SST are not acceptable)

Submission Container/Tube: Plastic vial

Specimen Volume: 1.0 mL

Collection Instructions: Within 2 hours of collection, centrifuge and aliquot serum into a plastic vial.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Red Refrigerated (preferred) 28 days
  Frozen  28 days
  Ambient  14 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Reference Values

Methadone:

Therapeutic: 100-400 ng/mL

Toxic: >2000 ng/mL

 

EDDP:

Not established

 

Cutoff concentrations by liquid chromatography tandem mass spectrometry:

Methadone: 10 ng/mL

EDDP: 5.0 ng/mL

Cautions

Methadone has a wide therapeutic index and dose-dependent toxicity. As a result, routine drug monitoring is not indicated in all patients.

 

Specimens collected in serum gel tubes are not acceptable because the drug can absorb on the gel and lead to falsely decreased concentrations.

Day(s) Performed

Tuesday, Thursday

Report Available

3 to 5 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

80299

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MDNS Methadone and Metabolite, S 96602-8

 

Result ID Test Result Name Result LOINC Value
36309 Methadone 3772-1
36310 EDDP 60071-8

NY State Approved

Yes

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

Forms

If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.

Secondary ID

36309