Test Code LAB10406 Blood Type & Coombs Direct
Performing Lab
Arnot Ogden Medical Center/ Ira Davenport Memorial Hospital
Ordering Recommendations
Determination of blood type and Rh.
Demonstrating in vivo coating of RBCs with IgG or the complement component C3d in the following settings:
-Autoimmune hemolytic anemia
-Hemolytic transfusion reactions
-Drug-induced hemolytic anemia
Collect
EDTA Whole Blood
Collection Device
EDTA Pink Tube or lavender microtainer for pediatric draws
Specimen Volume
5 ml
1 ml for pediatric patients
Stability (from collection to initiation)
Room Temp | 24 hours |
Refrigerated | 24 hours |
Minimum Requirements
3 mL
Methodology
Manual or Automated technology, Gel Column
Start Date
9/13/2017
Last Review Date
06/20/2025
7/11/25 AP
Specimen Handling
Refrigerated
Interpretive Data
A positive Direct Coombs indicates the red blood cells are coated with IgG or Complement. Both can lead to increased red blood cell destruction. Some medications or disease states can cause a positive Direct Coombs test.
Turn Around Time
STAT | 1 hour |
Routine | 8 hours |
*Additional testing may cause turn around time delays.
Reported
If the Direct Coombs, (IgG), is positive, an elution will be performed if the patient has been transfused in the past 3 months.