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Test Code LAB10396 Coombs Direct & Indirect

Performing Lab

Arnot Ogden Medical Center/ Ira Davenport Memorial Hospital

Ordering Recommendations

Demonstrating in vivo coating of RBCs with IgG or the complement component C3d in the following settings:

-Autoimmune hemolytic anemia

-Hemolytic transfusion reactions

-Drug-induced hemolytic anemia

Collect

Whole Blood

Collection Device

EDTA Pink Tube or lavender microtainer for pediatric draws

Stability (from collection to initiation)

Room Temp 24 hours
Refrigerated 3 days

Storage/Transport Temperature

Room Temp or Refrigerated

Specimen Handling

Refrigerated

Specimen Volume

5 mL

1 mL for pediatric draws

Minimum Requirements

3 mL

Remarks

TURN AROUND TIME

STAT 1 hour
Routine 8 hours

Methodology

Gel Column

Start Date

9/13/2017

Last Review Date

06/20/2025

7/11/25 AP